Boguski Proteomics Systems Biology

markboguski.net

Proteomics & Systems Biology

Identification of a Cytidine-Specific Ribonuclease from Chicken Liver (1980) |PubMed|PDF| I started out my research career as a cold-room enzymologist. Of course, these days, one would call this proteomics. RNase CL3 was originally isolated as a cytidine-specific RNase for use in RNA sequencing, analogous to Gilbert-Maxam DNA sequencing, but using base-specific enzymes rather than chemical cleavage. RNase CL3 was licensed to one of the earliest biotechnology companies, Bethesda Research Laboratories which has long since been acquired and merged out of existence. This enzyme is still in use as a research reagent more than 25 years after I first isolated and purified it (see examples).
Biosequence Exegesis (1999) |PubMed|PDF|
Biomedical Informatics for Proteomics (2003) |PubMed|PDF|
Genome Informatics: Current Status and Future Prospects (2003) |PubMed|PDF|
Systems Biology: Integrating in silico Approached and Experimental Approaches to Study Signaling Networks (2006) |PDF|

Also of note: transcript of an interview on the origins and clinical applications of proteomics

The scope of systems biology

Adapted from E.C. Butcher, et al. (2004) Systems Biology in Drug Discovery Nature Biotechnology 22:1253-1259